Inper offers a variety of implantable fiber optic cannula for optogenetics experiments. The fiber optic cannula consists of a ceramic or metal ferrule and a length of bare fiber. With the aid of a stereotaxic device, the fiber optic cannula can be implanted into the target nerve nuclei, and fixed on the skull with dental cement and screws. The fiber optic cannula can be connected to the optogenetic fiber via a matched mating sleeve to transmit the light from the LED or laser source to the target nerve nucleus, and then regulates the activity of neurons.
Diagram of Optogenetics
Two sizes of cannulas for meet different experimental requirements
Inper offers two sizes of fiber optic cannulas with Ø1.25 mm or Ø2.5 mm ferrules. The fiber optic cannula with Ø1.25mm ferrule has a small size and light weight, making it ideal for small laboratory animals, or multiple stimulation within a small area of an experimental animal. In contrast, the fiber optic cannula with Ø2.5mm ferrule is more conducive to surgical operation, and the connection between dental cement and experimental animals will also be stronger, suitable for large experimental animals, or a single point of stimulation.
Choice of NA
The numerical aperture (NA) is the ability of the end face of the fiber to accept light and also determines the angle at which the light is emitted from the fiber.
In experiments, the smaller the NA, the smaller the angle at which light is emitted from the fiber and the smaller the irradiated area, that is, the smaller the area of the nerve nuclei controlled by light. The larger the numerical aperture, the larger the angle at which light is emitted from the fiber and the larger the irradiated area, that is, the larger the area of the nerve nuclei controlled by light.
In optical fiber recording experiments, large numerical apertures can acquire more fluorescence signals.
Inper offers a variety of fiber options with different numerical apertures. The incident and emission angles for different NA are shown in the figure (Fiber-Air Interface).
High coupling efficiency
The fiber optic cannula are suggested to be connected to the matching optogenetic fiber provided by Inper. The light coupling rate can reach more than 85%. The consistency is also good. This ensures that the optical power at the ends of different ferrule needles from a single light source is very similar, which can minimizes the effect of fluctuations of optical power on the experimental results. Ultra-high light transmittance can also reduce the power requirements of the light source. Even the light from a single light source is split, it can still meet the power requirements for multiple stimulation.
Mating sleeve and optogenetic fiber compatibility
The matched mating sleeve should be employed to connect fiber optic cannula and optogenetic fiber The Ø1.25mm fiber optic cannula should be connected to optogenetic fiber using an ID 1.25mm mating sleeve, while the Ø2.5mm fiber optic cannula should be connected to optogenetic fiber with a an ID 2.5mm mating sleeve.
|Item||Compatible Ferrule Size||Length||Weight|
|MS125||1.25 mm Outer Diameter||6.8 mm||0.05 g|
|MS250||2.5 mm Outer Diameter||11.4 mm||0.18 g|
The standard length of the fiber of fiber optic cannula is from 0.5 to 40mm. For other customization, please contact email@example.com.