Fiber photometry system is a commonly used method for measuring calcium activity of neurons. It is characterized to record the activity changes of target neurons in special behavior through optical techniques. It can detect projection-defined activity signals in complex behaviors in real time，which is of great significance for studying the function of specific neural circuits in different behavioral paradigms.
With new types of fluorescent sensors for neurotransmitter coming out, the application range of photometry is further broadened, enabling real-time recording of dynamic changes of neurotransmitters (such as dopamine, acetylcholine, adenosine, etc) in specific brain regions during animal behavior. Fiber photometry system has become increasingly prominent as a tool for studying neurotransmitter release in vivo.
Inper have developed a new generation of multi-channel fiber photometry system in combination with epifluorescence microscopy, multimode fiber-conduction technology, and CMOS imaging, in response to the current the requirements of multi-sites and high-throughput calcium signal recording in neural circuits research. The system integrates ultra-stable LED light source, high-quality optical lens, high-sensitivity and low-noise CMOS chip, which can accurately collect the emitting fluorescence of target wavelength when the excitation light is output stably. Meanwhile, the system includes 6 TTL inputs for marking events, and 2 TTL outputs for exporting 5 V voltage signals once deltaF/F > preset value, which can meet the requirements of multiple event-related calcium signal acquisition in behavioral experiments.
The software for multi-channel photometry system has a concise interface that is easy to operate, and can simultaneously acquire signals and behavioral events. The signal sampling rate, exposure time of CMOS, and gain can be set, and signal previewed with software.