Inper Vista Multi-Channel Fiber Photometry System

Explore Inper Vista hardware, optics, Signal acquisition, behavior synchronization, optogenetic integration and Plot analysis, and choose a configuration for your experiment.

Explore configurations Discuss your experiment Fluorescence. In the context of behavior. Inper Vista Multi-Channel Fiber Photometry Multisite photometry. Bring signals, events and behavior video together. The host provides fluorescence excitation and detection and connects to the acquisition computer over USB. Choose the model around probes, fiber sites and stimulation needs. Vista host Fibers deliver excitation to implanted sites and return fluorescence to the instrument. Match patch cords, cannulas and sleeves to fiber cores, interfaces and the experiment. Fibers and connectors Signal manages acquisition settings, live signals, video and events. Plot processes recorded data, organizes events and produces analysis figures. A computer is supplied separately. Signal and Plot The fiber path carries excitation and returning fluorescence. Confirm the host interface and fiber specification, and keep end faces clean and connections secure. Photometry fiber Eight DIO lines are configured individually as inputs or outputs. Inputs receive experimental events; outputs communicate start, stop or conditional events to other equipment. Digital I/O Analog inputs connect through the expansion box. Same-site stimulation requires a compatible model and optogenetic equipment. Diagrams explain functional connections; physical interfaces follow the selected model. Expansion and synchronization Host, fibers and software. A purpose for every connection. The system Excitation travels through the optical assembly and fiber to fluorescent probes in tissue. Emitted fluorescence returns through the fiber, is separated by wavelength and reaches the camera. Software extracts fluorescence intensity from end-face ROIs to form time-series records. Excitation, transmission and detection. Functional schematic of excitation and fluorescence collection. How it works Check excitation, emission collection bands and host model together. The use of a reference channel depends on the probe and experimental design. Use as a reference acquisition channel for suitable probes. Fit and subtract reference signals according to experimental conditions and retain raw signals for review. 410 nm Reference excitation The source specification pairs 470 nm excitation with a 503–538 nm collection band. Available combinations depend on the model and probe spectrum. 470 nm Green fluorescence recording The source specification pairs 561 nm excitation with a 582–619 nm collection band. Choose a red channel or a green/red combination for the experiment. 561 nm Red fluorescence recording Vista 410 adds 640 nm to the excitation combination. Confirm probe compatibility, acquisition configuration and operating conditions for the intended experiment. 640 nm · Vista 410 An additional band on Vista 410 Match the optics to the probe. Optical configuration The camera images fiber ends; software extracts population fluorescence from each ROI. Arrange sites, naming and sampling for the fibers and experiment. The camera images the fiber ends, and software extracts fluorescence intensity from each ROI. Fiber sites, wavelength count and sampling rate per wavelength together define the acquisition setup. Relate each fiber end in the camera image to its signal trace. Camera detection Fiber-end images and signal traces Connect multiple recording sites with a suitable branched fiber. Record multiple brain regions in one animal, or configure recordings across animals. Software assigns separate end-face ROIs with individual traces and names. Site counts depend on model, core diameter and fiber assembly. Fiber sites and excitation wavelengths are different dimensions. Documented combinations include about 9 sites with 200 μm cores or 4 with 400 μm cores, subject to model and fiber specification. Multiple sites Distinguish sites and wavelengths Detect fiber ends in the camera field and create ROIs for fluorescence extraction. Check the position and useful area of each end face before recording. Automatic detection Automatic detection Add, remove or adjust ROIs to match the actual fiber ends. Check the regions again after changing fibers or connections. Manual adjustment Manual adjustment Use recognizable ROI names for animals, brain regions or recording sites. End-face detection establishes acquisition regions; probe expression is confirmed separately. Site names Site names Multiple sites. Identify and record each one. Detection and recording sites Select excitation bands, then coordinate power, exposure, sampling rate and gain. Preview signals before recording and confirm settings against measured optical power and experimental conditions. Select the wavelengths for the experiment and set their power individually. Measurement mode supports output checks with a power meter; experiment mode follows the acquisition settings. Excitation Select the excitation bands Exposure and per-wavelength sampling determine acquisition timing. Recheck total camera frame rate when adding wavelengths; individual limits do not describe one simultaneous operating setup. Exposure and sampling Coordinate the light and camera timing Gain amplifies noise as well as signal. Use Preview to inspect end faces and traces before recording and saving the experiment. Gain and preview Preview before recording Configure acquisition for the experiment. Prepare to record View signals, events and video in one interface. Choose recording modes for the experiment and inspect raw and processed traces. View fluorescence traces, event markers and behavior video in the acquisition interface. Adjust plot ranges and time windows, inspect settings in Preview, then save data and enabled video recordings when the experiment starts. Live interface Inper Signal. See the recording as it happens. Continuous mode records without planned gaps. Interval mode follows configured illumination durations and pauses. It reduces continuous illumination; Plot can retain the valid acquisition segments for processing. Choose recording duration and illumination around the probe, animal and protocol. Continuous and interval Record continuously. Or at planned intervals. Inspect changes in fluorescence and reference channels alongside fiber images, optical power and behavior to identify segments that need review. Signal and reference Inspect the source channels Configure subtraction, reference correction or ΔF/F calculations with the required signal, control and calculation window. Live calculations View calculated traces alongside recordings Configure filtering, smoothing, offsets and axis ranges. Combined and separate axes help inspect relationships between channels. Display and processing Set the view for the signal Inper Signal. See the recording as it happens. Live acquisition Record behavior video and enable single-mouse tracking and zone events when needed to review behavior alongside fluorescence. Inper Signal records behavior using connected, enabled USB cameras. View video with fluorescence traces to inspect what happened around an event, then continue synchronized review in Plot after acquisition. Behavior video Behavior on screen. Fluorescence alongside it. With Animal Tracking enabled, software detects the position of a single mouse. Draw rectangular zones and configure entry, exit or dwell-time conditions for markers or outputs. Recorded tracking helps review the animal’s relationship to these zones. Position and zones Enter, leave or stay. Use zone events in the experiment. Behavior and signals. In the context of the same experiment. Behavior and zone events Turn experimental actions, external equipment signals and signal conditions into markers for review, segmentation and event alignment. Use keyboard shortcuts or acquisition start/stop events. Name and color markers to distinguish experimental actions. The video shows an original acquisition and marking demonstration. Manual and start/stop Mark experimental actions Configure the relevant DIO line as an input to receive equipment events through TTL. Match I/O allocation to the physical setup. External TTL Bring equipment events into the recording Set ΔF/F conditions, a calculation window and a refractory period for ROI or analog data. Define trigger conditions in the settings interface and check the event logic before the experiment. Fluorescence threshold Mark a configured signal condition Keep the key moments in the recording. Experimental events Use DIO for digital events and an external ADC for continuous analog signals. Configure each connection for experimental timing and electrical conditions. Assign digital lines to receive external events or output start, stop, manual and conditional events to other equipment. Keep each line’s allocation consistent with its connection. Receive external TTL and include events from stimulators or behavior equipment in the recording. Send acquisition start/stop, shortcut or configured signal-condition events to external equipment. Configure immediate, delayed, scheduled or external-triggered start/stop for the protocol. 8 DIO lines 8 DIO lines. Assign each an input or output. Connect analog outputs from external equipment through the ADC expansion box. Activate, name and view analog channels in Signal. The specification lists an eight-input configuration with up to 30 kHz per channel. Confirm the expansion box, input electrical conditions and sampling settings for the experiment. ADC analog expansion Record analog signals alongside fluorescence. Digital and analog. Connect each signal appropriately. External signals Compatible optogenetic models use Stimulus settings to place stimulation within the available gaps between fluorescence excitation and exposure. Set sampling and exposure first, then configure pulse width, frequency and waveform sequences with matched optogenetic equipment. One site. Coordinate stimulation and acquisition. Alternating timing schematic. Available gaps and pulse widths depend on sampling and exposure settings. Same-site stimulation Save and import Config files for later experiments. Save the configuration Configuration files preserve acquisition settings for reuse. Check them against the current host, fibers, excitation and connections before using them in another experiment. Use Note for treatments, observations and items to revisit. Add notes during recording Add notes during acquisition to provide context for review. Keep animal information, treatments and observations consistent with the experiment’s records. Pause the plot view, inspect an earlier segment and return to the live position. Review earlier signals Inspect earlier traces with the plot’s time controls, adjust the time window and switch between combined or separate axes. The display view and data recording follow their respective software states. Keep the experiment’s context. Bring Inper Signal recordings into a Plot project to review signals, event markers and behavior video. Select analysis windows, calculate ΔF/F and Z-scores, and display traces, averages and heatmaps. Edits within the project preserve the original recordings. Signals, heatmaps and statistics. In one project. Actual interfaces and example data from the Inper Plot 1.8.0 manual, shown to illustrate software functions. Choose processing steps for the characteristics of each recording and name the resulting data. Separate tools address offsets, noise, slow baseline trends and interval recordings. The researcher controls the sequence and parameters. Offset subtracts a specified value from the signal. For control-based correction, choose Signal and Control, match them using addition or multiplication, and subtract the control contribution into a processed data channel. Offset and background Choose what to subtract. FIR filtering provides band-pass and band-stop settings; smoothing offers median and mean methods. Cutoff frequencies must suit the sampling rate, while the researcher chooses window sizes and processing strength. Filtering and smoothing Set processing parameters for the data. Fit the signal trend with a polynomial and apply baseline correction. Choose the polynomial order, inspect the curves and adjust parameters for the experiment. This processing step is separate from selecting F₀ for ΔF/F. Baseline correction Correct slow baseline trends. For original interval recordings, Delete interval removes gaps while retaining a chosen duration from each recording segment. Optional baseline correction can be applied to the retained segments before further analysis. Interval recordings Retain the recording segments to analyze. Prepare the signal before analysis. Preprocessing ΔF/F depends on how F₀ is selected. Plot offers three baseline modes to suit experimental events and analysis windows. For Z-scores, select the interval used to calculate the reference mean and express signal changes relative to it. Choose a signal interval using the recording’s absolute time and use its mean as a fixed F₀. This suits analyses with a defined reference interval. Set the F₀ interval relative to each Marker, linking the baseline window to its corresponding experimental event. Calculate ΔF/F with a dynamic baseline. Set the related parameters for the sampling rate and signal characteristics, then inspect the calculated result. Define the reference before comparing signals. ΔF/F and Z-scores Add, shift or import events in the project, then review video and trim related data to prepare corresponding analysis segments. Add a Marker at a cursor position or entered time, and remove unwanted markers. Keep the events needed for alignment and analysis. Add and remove Add and remove Select markers and enter a positive or negative offset to shift them later or earlier together. The researcher sets the offset explicitly. Shift in time Shift in time Create an empty event channel or import time entries from an Excel sheet in the required format to add external events to the project. Import external events Import external events Play a behavior video on its own or review it alongside selected signals. Playback supports 0.5×, 1×, 1.5× and 2× speeds, plus forward and backward steps of a chosen duration. When retaining a segment, select the corresponding signals for linked trimming. Video and linked trimming Return to the moment of the behavior. Organize events. Return to the behavior. Organize events and review Define the time range for each trial, then organize the data for analysis. Review segments relative to experimental events and add selected data to Groups to plot individual traces, group averages and heatmaps. With Marker defines each trial window relative to its event. With Specific Region selects intervals using the recording’s absolute time. Set trial and baseline ranges explicitly for the analysis. Define trials Create Groups and add the data channels to compare. Group definitions are saved with the project so the same organization can be used to review traces, averages and heatmaps later. Organize Groups Compare recordings around the same event. Event alignment and grouping Use the selected analysis data and define the time interval and reference line before calculating. Find fluorescence peaks using a selected interval and threshold, or define event intervals by relative change and duration. The researcher sets the detection criteria; results support organization by count, frequency and amplitude. Calculate the full area, positive area or negative area. Use zero or the mean of a specified interval as the reference line so the basis of each calculation is explicit. AUC: area within an interval AUC: area within an interval Find the peak within the selected interval and report its value and timing for comparison with events and other trials. Peak: value and timing Peak: value and timing Inspect trough values and their timing within the same analysis framework. Export the statistics as a table for further organization and analysis. Trough: value and timing Trough: value and timing Choose the search interval and set thresholds and a window using information such as MAD. Inspect detected peaks and export values, times, counts and frequency. Here, Spike refers to peaks in the fluorescence trace. Spike peaks Find transient peaks with explicit thresholds. Choose a reference interval for F₀ and set a relative-change threshold and minimum duration. Qualifying intervals are highlighted on the trace to review their distribution and frequency. Event Frequency Identify intervals of sustained change. Area, extrema and events. Turn traces into results. Curve statistics and transient events Export analysis figures as SVG vectors or PNG images. Signals, events and statistics can be exported as CSV or Excel tables through their respective tools. Before export, adjust plot axes and heatmap color ranges, and select the data segments to retain. Take figures and data into the next step. Figure and data export Researchers related GCaMP6s fluorescence to behavioral events and compared responses around those events. Neural activity and behavior Read the methods Calcium responses during stress Yang et al., Nature (2025), Stress dynamically modulates neuronal autophagy to gate depression onset. Methods describe an Inper photometry system and processing software used with MATLAB, reference correction, event segmentation, ΔF/F and AUC analysis. DOI: 10.1038/s41586-025-08807-4. Multichannel photometry was used to observe fluorescent sensor dynamics under probe-specific behavioral protocols. Multicolor photometry Read the methods In-vivo validation of neuropeptide sensors Wang et al., Science (2023), A tool kit of highly selective and sensitive genetically encoded neuropeptide sensors. Methods identify an Inper OPT-FPS-410/470/561 multichannel photometry device. The cited device name does not establish every capability of current Vista models. DOI: 10.1126/science.abq8173. Define probes, sites, behavioral events and analysis methods, then confirm the hardware and software combination. Methods and configuration Configure around the research question Publications show the methods actually used in a study. Match their probes, optical settings, equipment and processing steps to the new experiment, and confirm the current model and software version. Recording methods in published research. Select for interfaces, fiber specifications and workflow. Included and optional items follow the confirmed delivery list. Connect the host to implanted sites with matched branching, core diameter and interfaces. Low-autofluorescence fibers Connect implanted ends to patch cords with matching dimensions and holders. Cannulas and sleeves Exclude stray ambient light to record and inspect dark-condition offset references. Dark box Add analog inputs and connections when the experiment requires external analog signals. ADC expansion box Use voltage conversion or divide-by-two mode as required by external equipment signals and event connections. Voltage conversion box A purpose for each accessory. Excitation wavelengths / nm Optogenetic integration Confirm model capability Confirm model capability Compatible configuration Compatible configuration Confirm configuration Sampling configuration Set for excitation and exposure Set for excitation and exposure Set for excitation and exposure High-frame-rate model; confirm conditions Set for excitation and exposure Before choosing Probes and sites Multicolor setup and sites Stimulation and timing Wavelengths, exposure and frame rate Four-color probes and acquisition Choose Vista around the experiment. Inper Plot User Manual 1.8.0 Read operating requirements, functions and configuration conditions for the relevant version. Prepare the computer and external equipment for the experiment. Inper Signal User Manual 2.0.0 Read operating requirements, functions and configuration conditions for the relevant version. Prepare the computer and external equipment for the experiment. Inper Vista Product Brochure Read operating requirements, functions and configuration conditions for the relevant version. Prepare the computer and external equipment for the experiment. Inper Product Catalog 2026.B Read operating requirements, functions and configuration conditions for the relevant version. Prepare the computer and external equipment for the experiment. Resources and preparation. Detection sCMOS camera; fluorescence extracted from fiber end-face ROIs Models and excitation wavelengths Vista 210: 410 / 561 nm; 310, 320, 320P: 410 / 470 / 561 nm; 410: 410 / 470 / 561 / 640 nm Fluorescence collection bands 470 nm excitation: 503–538 nm; 561 nm excitation: 582–619 nm. Confirm other bands for the configuration. Example fiber-site configurations About 9 sites with 200 μm cores or 4 with 400 μm cores, subject to model and fiber assembly. Documented sampling frame rate 1–300 fps; high-frame-rate Vista 320P up to 500 fps. Confirm the actual per-wavelength sampling rate against wavelength count and exposure. Exposure and gain Exposure 1–100 ms; gain 1–24×. Available combinations follow the sampling configuration. Recording modes Continuous / Interval Digital I/O 8 lines, individually configured as inputs or outputs Analog expansion External ADC expansion box: 8 inputs, up to 30 kHz per channel; confirm electrical conditions and sampling settings. Same-site stimulation Vista 320 / 320P support compatible optogenetic configurations; coordinate stimulation pulse width with exposure and sampling timing. Software Inper Signal acquisition / Inper Plot analysis; use the relevant software version Computer and external equipment Computer supplied separately; prepare cameras, stimulation equipment and expansions according to the confirmed configuration. System specifications and configuration conditions Parameter Value / description These are documented ranges and configuration examples. Individual limits do not describe one simultaneous operating setup. Confirm wavelength count, exposure, camera frame rate and sampling per wavelength together; delivery follows the agreed model and configuration list. Technical specifications Share your research focus, experimental subjects and configuration needs for product selection guidance. Ask about Inper Vista Multi-Channel Fiber Photometry System Send inquiry Product selection Configuration and quotation Technical and application support