Fiber photometry system is a commonly used method for measuring calcium activity of neurons. It is characterized to record the activity changes of target neurons in special behavior through optical techniques. It can detect projection-defined activity signals in complex behaviors in real time，which is of great significance for studying the function of specific neural circuits in different behavioral paradigms.
410nm Helps Remove Noise Signlas
In the fiber photometry experiment, various kinds of artificial noise may affect the accuracy of experimental result. These noise includes autofluorescence caused by tissue damage, motion-induced changes caused by free-moving animals, photobleaching induced by long-term imaging, and other false positive signals interference caused by non-calcium concentration changes. However, the traditional fiber photometry recording solution cannot evaluate and reduce the impact of the artificial noise on the experimental results. When the calcium sensor is excited by light from 405 to 420 nm, its emission fluorescence intensity maintains a constant state with the change of intracellular Ca2+ concentration. Therefore, to some extent, it can be considered that the fluorescence signal excited at 410 nm reflects the noise signal except for the change of the Ca2+ concentration. On the basis of the principle above, 410 nm light can be added as control in fiber photometry system. The fluorescence signal corresponding to exciting light 410 nm, and 488 nm (GCaMP)/561 nm (RCaMP) will be simultaneously recorded. During fluorescence signal analysis, this system can effectively remove noise signals and greatly improve the accuracy of experimental data.
Highly configurable IO and Colors
Inper have developed a new generation of multi-channel fiber photometry system in combination with epifluorescence microscopy, multimode fiber-conduction technology, and CMOS imaging, in response to the current the requirements of multi-sites and high-throughput calcium signal recording in neural circuits research. The system integrates ultra-stable LED light source, high-quality optical lens, high-sensitivity and low-noise CMOS chip, which can accurately collect the emitting fluorescence of target wavelength when the excitation light is output stably. Meanwhile, the system includes 6 TTL inputs for marking events, and 2 TTL outputs for exporting 5 V voltage signals once ΔF/F > preset value, which can meet the requirements of multiple event-related calcium signal acquisition in behavioral experiments
Stable LED with 410, 470, 561, 635 nm
High sensitivity and low noise CMOS chip
High quality filter lenses
Rich digital I/O
Powerful Recording Software With Well-designed Interface
The software for multi-channel photometry system has a concise interface that is easy to operate, and can simultaneously acquire signals and behavioral events. The signal sampling rate, exposure time of CMOS, and gain can be set, and signal previewed with software.
Professional Data Analysis of Visualization
Without any programming knowledge, you can add or delete the marker points at will by dragging the mouse, and analyze single or multiple groups of experimental data.
The analysis software Can directly generate commonly used DeltaF / F, Z-Score, Heatmap, and other figures, you can also export the original data or processed data to CSV or other formats for processing with other data analysis software.
All images are exported in vector format. You can directly edit the software with Illustrator, CorelDRAW and other software to change the color and fonts.
Specifications
| Optic Fiber Connector | FC or SMA 1.9 mm tailstock Autofluorescence |
| Excitation Wavelength | 410 nm, 470 nm, 561 nm |
| Optical Power | 0-120 μW |
| Power adjustment | Software |
| Power accuracy | 0.1 μW |
| Acquisition mode | Continuous or Sequential |
| Sampling | 1 - 320 FPS |
| Exposure time | 1 - 100 ms |
| Number of Channels | 9 in default and more available for customization. Simultaneous Recording in three colors |
| Connector Type | Inputs: 6 Outputs: 2 |
| Connection mode | USB 3.0 |
| Markers | Auto marking based on pre-set threshold of ΔF/F Manual |
| Output | ＞ ΔF/F threshold At start of recording At end of recording |
| Adaptation adjustment | Free adjustment of fiber end face Free configuration of ROI |
| File | Customized file name and path |
| Acquisition interface | Solid or auto timeline |
| Software | Recording and analysis Vector format Customization |
| Analysis | Algorithm integration Free choice of control channel Export of ΔF/F raw data Offline Marking Synchronization with other physiological signal |
| Video | Integration of behavior video recording |
Specifications for 光纖記錄儀
| 激發光波長（nm） | 410 or 470 or 561 or 410/470 or 410/561 or 470/561 or 410/470/561 |